The problem with traditional microarrays
Multiplexed sandwich immunoassays is a powerful technique to measure multiple protein concentrations simultaneously. Despite a great initial excitement, its success was hampered by cross-reaction, which results in false positive signals. Cross-reaction occurs because the detection antibodies are mixed together when applied to the array. Moreover, it is often impossible to combine related analytes in the same assay due to incompatibility.
The SnapChip Solution
The SnapChip developed by Parallex BioAssays is an innovative and simple microarray-to-microarray approach that eliminates the need for a mixture of detection antibodies and the related cross-reactivity. The absence of cross-reaction is highly desirable to reduce assay development time and cost, ensure accurate results and discover new opportunities. The SnapChip reproduces, in nanodroplets, the conditions of the common ELISA by performing colocalization of the capture and detection antibodies. It’s performed on standard planar arrays, representing an attractive solution for scientists to accelerate their research without investing in expensive equipment.
The SnapChip is more than a simple multiplex, it’s multiple singleplex!
In the SnapChip microarray-to-microarray approach, the colocalization of capture and detection antibodies efficiently eliminates cross-reaction as detection antibodies are physically isolated from each other. It is also highly customizable and there are no incompatibility issues, as no analytes are mixed together. As a result, the assay optimization process is simplified.
Advantages of the SnapChip
- Rapid Assay Development – All assays are optimized individually. That’s it!
- Mix and Match – All your preferred assays on the same chip. No limitation!
- Accurate Results – Elimination of cross-reactivity and false positives!
- New Opportunities – Measurement of concentration, post-translational modifications and enzyme activity… All at once!
SnapChip assay process flow
The assay process flow using the SnapChip is highly similar to a regular multiplexed sandwich assay on a planar microarray. Samples are first incubated on the assay slide containing an assembly of independent and parallelized capture antibodies, physically isolated from each other. After wash steps, biotinylated-detection antibodies from the pre-spotted detection slide are precisely delivered to their corresponding spots on the assay slide using the SnapDevice. Following proper incubation and washing, the assay signal is created by incubation with fluorescent-labelled streptavidin and fluorescent signals are revealed by a scanner with fluorescent capability.
Antibody Colocalization Microarray: A Scalable Technology for Multiplex Protein Analysis in Complex Samples (MCP, 2012)
Pla-Roca M, Leulmi RF, Tourekhanova S, Bergeron S, Moreau E, Laforte V, Goselin S, Bertos N, Hallett M, Park M, Juncker D