Parallex BioAssays

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The problem with traditional microarrays

Multiplexed sandwich immunoassays is a powerful technique to measure multiple protein concentrations simultaneously. Despite a great initial excitement, its success was hampered by cross-reaction, which results in false positive signals. Cross-reaction occurs because the detection antibodies are mixed together when applied to the array. Moreover, it is often impossible to combine related analytes in the same assay due to incompatibility.

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The SnapChip Solution

The SnapChip developed by Parallex BioAssays is an innovative and simple microarray-to-microarray approach that eliminates the need for a mixture of detection antibodies and the related cross-reactivity. The absence of cross-reaction is highly desirable to reduce assay development time and cost, ensure accurate results and discover new opportunities. The SnapChip reproduces, in nanodroplets, the conditions of the common ELISA by performing colocalization of the capture and detection antibodies. It’s performed on standard planar arrays, representing an attractive solution for scientists to accelerate their research without investing in expensive equipment.

The SnapChip is more than a simple multiplex, it’s multiple singleplex!

In the SnapChip microarray-to-microarray approach, the colocalization of capture and detection antibodies efficiently eliminates cross-reaction as detection antibodies are physically isolated from each other. It is also highly customizable and there are no incompatibility issues, as no analytes are mixed together. As a result, the assay optimization process is simplified.

Advantages of the SnapChip

  • Rapid Assay Development - All assays are optimized individually. That’s it!
  • Mix and Match - All your preferred assays on the same chip. No limitation!
  • Accurate Results - Elimination of cross-reactivity and false positives!
  • New Opportunities - Measurement of concentration, post-translational modifications and enzyme activity… All at once!

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SnapChip assay process flow

The assay process flow using the SnapChip is highly similar to a regular multiplexed sandwich assay on a planar microarray. Samples are first incubated on the assay slide containing an assembly of independent and parallelized capture antibodies, physically isolated from each other. After wash steps, biotinylated-detection antibodies from the pre-spotted detection slide are precisely delivered to their corresponding spots on the assay slide using the SnapDevice. Following proper incubation and washing, the assay signal  is created by incubation with fluorescent-labelled streptavidin and fluorescent signals are revealed by a scanner with fluorescent capability.

SnapDevice

    • No cross-reaction
    • Spotter free
    • Multiparametric
    • Custom
    • Reliable

      Publications

      Immunohistochemistry Microarrays

      Huiyan Li, Gabrielle Brewer, Grant Ongo, Frederic Normandeau, Atilla Omeroglu and David Juncker

      Antibody Colocalization Microarray for Cross-reactivity-Free Multiplexed Protein Analysis

      Véronique Laforte, Pik-Shan Lo, Huiyan Li and David Juncker

      Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays (J. Vis. Exp.,2017)

      Li H, Bergeron S, Larkin H, Juncker D

      A versatile snap chip for highdensity sub-nanoliter chip-to-chip reagent transfer (Scientific Reports, 2015)

      Li H, Munzar JD, Ng A, Juncker D

      Serial analysis of 38 proteins during the progression of human breast tumor in mice using an antibody colocalization microarray (MCP, 2015)

      Li H, Bergeron S, Annis MG, Siegel PM, Juncker D

      Cross-reactivity in antibody microarrays and multiplexed sandwich assays: shedding light on the dark side of multiplexing (CurrOpinionChemBio, 2014)

      Juncker D, Bergeron S, Laforte V, Li H

      Microarray-to-Microarray Transfer of Reagents by Snapping of Two Chips for Cross-Reactivity-Free Multiplex Immunoassays (AnalChem, 2012)

      Li H, Bergeron S and Juncker D

      Antibody Colocalization Microarray: A Scalable Technology for Multiplex Protein Analysis in Complex Samples (MCP, 2012)

      Pla-Roca M, Leulmi RF, Tourekhanova S, Bergeron S, Moreau E, Laforte V, Goselin S, Bertos N, Hallett M, Park M, Juncker D

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